LLC

Stomata

Stomata are of great interest for a variety of reasons. Some scientists are comparing stomata in recent and fossil leaves in order to better understand the importance of global warming1, 2. Others propose manipulating stomatal function using combinations of physiological and molecular genetic methods in order to improve water use efficiency of plants.3 This is especially important in the case of droughts. Plants are classified by the sizes, shapes, arrangements, and densities of stomata on leaves.4 Understanding the role and behavior of stomata provides guidance that can lead to improvements in foliar (leaf-applied) fertilization.5 The study of stomatal physiology can help in the discovery of new strategies to prevent pathogens from entering leaves.6 The locations of stomata on leaves can reduce water loss that can lead to fire risk. This can lead to planting of certain kinds of trees in forested areas that are subject to periodic wildfires.7 Detailed microtopographic images like those below can provide guidance for these and other purposes.

Stomata are found on the upper and lower sides of leaves, on flower petals, stems, and on roots. Scientists survey plant surfaces to determine the density and size of stomata and relate these findings to properties of the environment, such as the temperature and amounts of sunlight, humidity, oxygen and carbon dioxide in the air when a leaf is formed. Leaves of terrestrial plants generally have more stomata on their undersides. Floating leaves of aquatic plants generally have stomata only on their upper surfaces while some underwater plants have no stomata at all. The seeds of monocots (grasses, bulbs) have a single embryonic leaf or cotyledon. The major veins in monocots are parallel to the leaf axis. Their stomata are generally oriented similarly to one-another. Dicot seeds (oak trees, roses) have two embryonic leaves. Their leaf veins are in net-like arrangements. They have stomata that are randomly oriented. These differences are seen in the images below.

Specimen preparation

Epidermal Peel Epidermal Shave Epidermal Cast

These preparation methods are well known. There are many examples on the Internet. All specimens were wet-mounted. A homemade microtome was used for cutting wax-embedded leaf cross-sections.8

Equipment

A Nikon Eclipse LV100 microscope and a Point Grey Grasshopper camera were used. Koehler illumination was used throughout. Image processing and focus stacking software were used where appropriate.

Images

Each set of images includes a picture of a plant, magnified views of stomata, a stereo close-up, a dynamic perspective view, and a cross-sectional view of a stoma. The plants shown here all grow in San Francisco, California.

Click on an image to enlarge it.

Agave hybrid 'Blue Glow'.

Agave 'Blue Glow' is a succulent, monocarpic monocot. The leaf axis is vertical in these images.

 

Beschorneria yuccoides 'Flamingo Glow'.

B. yuccoides is a succulent monocot in the family Asparagaceae, subfamily Agavoideae. The leaf axis is vertical in these images.

 

Podophyllum pleianthum.

P. pleianthum is an herbaceous perennial dicot.

 

Masdevallia Highland Monarch 'Golden Sunshine'.

Masdevallia Highland Monarch 'Golden Sunshine' is a monocot.

 

Clivia miniata, yellow form.

C. miniata is a monocot. The leaf axis is vertical in these images.


Time-Lapse Videos

Tradescantia zebrina

This is a 1.5 hour time-lapse video of a stoma on a Tradescantia zebrina leaf. Image width is 250 microns.
An image was taken every 2 minutes. The sequence reverses half way through the video.

   

Salvia microphylla var. "Hot Lips"

This is a 14 minute time-lapse video of stomata on a Salvia microphylla leaf. Image width is 622 microns.
An image was taken every 2 minutes. The sequence reverses half way through the video.